105545 serotype aav9 Search Results


egfp  (Addgene inc)
95
Addgene inc egfp
The effect of genotype on the pain sensitivity determined by tail-flick test ( A , B ). The influence of genotype ( A ) and the influence of AAV-Cre injection ( B ) on thermal pain sensitivity. Data are the mean ± SEM. n = 5–14 ( A ), n = 8 ( B ). +/+, wild type; +/flox, floxed hetero; flox/flox, floxed homo. The effect of knockdown of RTP4 <t>in</t> <t>PVN</t> on the development of antinociceptive tolerance to morphine ( C ). The daily changes in antinociceptive effect of morphine during repeated administration. Data are the mean ± SEM. n = 7 <t>(AAV-eGFP)</t> (control), n = 10 (AAV-Cre), **** p < 0.0001 vs. Day1 (AAV-eGFP); # p < 0.05, #### p < 0.0001 vs. Day1 (AAV-Cre), Dunnett’s multiple comparison test; † p < 0.05, †† p < 0.01 vs. AAV-eGFP (control), repeated measures ANOVA and the post-hoc Tukey’s multiple comparisons test. The effect of AAV-Cre on RTP4 expression in PVN area (D and E). Representative images of the immunohistochemical signal for RTP4 and the AAV-eGFP signal of the virus infected in the PVN area. Arrows indicate the eGFP-positive cell. The mean intensity of the RTP4 signal in eGFP-positive cells were measured by Image J software, as described in the Methods section (E). The mean signal intensity of RTP4 in the RTP4/eGFP double-positive cells were measured from a single cryostat brain section, including the PVN region from 7 (AAV-eGFP) and 6 (AAV-Cre) mice. Ten to 40 eGFP-positive cells were measured from each section. Data are the mean ± SEM. n = 7 (AAV-eGFP) (control), n = 6 (AAV-Cre), * p < 0.05 unpaired t -test.
Egfp, supplied by Addgene inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/105545+serotype+aav9/pmc09599210-42-18-21?v=Addgene+inc
Average 95 stars, based on 1 article reviews
egfp - by Bioz Stars, 2026-08
95/100 stars
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astrocyte specific gfaabc1d promoter  (Addgene inc)
93
Addgene inc astrocyte specific gfaabc1d promoter
Expression of FABP5 in astrocytes of FABP5 KO mice rescues DSI (A) AAV-mediated expression of FABP5 under the control of the <t>gfaABC1D</t> promoter in FABP5 KO mice. a1-a3, Immunostaining of FABP5, s100β and merge. Scale bar: 50 μm. a4, Co-localization of FABP5 with s100β. a5, Lack of co-localization of FABP5 with NeuN. Scale bars: 10 μm. (B) Expression of FABP5 in astrocytes rescues DSI in FABP5 KO mice. Left panel, averaged magnitude of DSI obtained in WT ( : 42.23 ± 3.44%; n = 12 cells; N = 3 mice), KO ( : 2.65 ± 1.03%; n = 13 cells; N = 3 mice; p = 3.28×10 −13 versus WT), KO+AAV-gfaABC1D-FABP5 ( : 33.37 ± 3.50%; n = 13 cells; N = 4 mice; p = 0.12 versus WT), and KO+AAV-GFAP-EGFP ( : 6.07 ± 1.58%; n = 12 cells; N = 4 mice; p = 1.71×10 − 8 versus KO+AAV-gfaABC1D-FABP5). Right panel, IPSC traces collected before and during DSI from WT ( ), KO ( ), KO+AAV-gfaABC1D-FABP5 ( ), and KO+AAV-GFAP-EGFP ( ). Scale bars: 100 ms, 200 pA. (C) AAV-mediated expression of FABP5 under the neuron-specific hsynapsin1 promoter in FABP5 KO mice. c1-c3, Immunostaining for NeuN, FABP5, and merge. Scale bar: 50 μm. c4, Co-localization of FABP5 with NeuN. c5, Lack of co-localization of FABP5 with s100β. Scale bars: 10 μm. (D) Expression of FABP5 in neurons partially rescues DSI in FABP5 KO mice. Left panel, Summary of DSI magnitude recorded from WT ( : 39.52 ± 1.65%; n = 11 cells; N = 5 mice), KO ( : 1.43 ± 0.66%; n = 11 cells; N = 4 mice; p = 4.67×10 −18 versus WT), KO+AAV-hSyn-FABP5 ( : 13.95 ± 2.13%; n = 15 cells; N = 5 mice; p = 4.47×10 −13 versus WT) and KO+AAV-hSyn-EGFP ( : 5.15 ± 1.61%; n = 12 cells; N = 4 mice; p = 0.89 versus KO). Right panel, IPSC traces collected before and during DSI. Data are represented as mean ± SEM. ∗ p < 0.05; ∗∗∗ p < 0.001, one-way ANOVA with Bonferroni’s multiple comparisons test.
Astrocyte Specific Gfaabc1d Promoter, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/105545+serotype+aav9/pmc12033926-320-72-81?v=Addgene+inc
Average 93 stars, based on 1 article reviews
astrocyte specific gfaabc1d promoter - by Bioz Stars, 2026-08
93/100 stars
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aav cre  (Addgene inc)
96
Addgene inc aav cre
Expression of FABP5 in astrocytes of FABP5 KO mice rescues DSI (A) AAV-mediated expression of FABP5 under the control of the <t>gfaABC1D</t> promoter in FABP5 KO mice. a1-a3, Immunostaining of FABP5, s100β and merge. Scale bar: 50 μm. a4, Co-localization of FABP5 with s100β. a5, Lack of co-localization of FABP5 with NeuN. Scale bars: 10 μm. (B) Expression of FABP5 in astrocytes rescues DSI in FABP5 KO mice. Left panel, averaged magnitude of DSI obtained in WT ( : 42.23 ± 3.44%; n = 12 cells; N = 3 mice), KO ( : 2.65 ± 1.03%; n = 13 cells; N = 3 mice; p = 3.28×10 −13 versus WT), KO+AAV-gfaABC1D-FABP5 ( : 33.37 ± 3.50%; n = 13 cells; N = 4 mice; p = 0.12 versus WT), and KO+AAV-GFAP-EGFP ( : 6.07 ± 1.58%; n = 12 cells; N = 4 mice; p = 1.71×10 − 8 versus KO+AAV-gfaABC1D-FABP5). Right panel, IPSC traces collected before and during DSI from WT ( ), KO ( ), KO+AAV-gfaABC1D-FABP5 ( ), and KO+AAV-GFAP-EGFP ( ). Scale bars: 100 ms, 200 pA. (C) AAV-mediated expression of FABP5 under the neuron-specific hsynapsin1 promoter in FABP5 KO mice. c1-c3, Immunostaining for NeuN, FABP5, and merge. Scale bar: 50 μm. c4, Co-localization of FABP5 with NeuN. c5, Lack of co-localization of FABP5 with s100β. Scale bars: 10 μm. (D) Expression of FABP5 in neurons partially rescues DSI in FABP5 KO mice. Left panel, Summary of DSI magnitude recorded from WT ( : 39.52 ± 1.65%; n = 11 cells; N = 5 mice), KO ( : 1.43 ± 0.66%; n = 11 cells; N = 4 mice; p = 4.67×10 −18 versus WT), KO+AAV-hSyn-FABP5 ( : 13.95 ± 2.13%; n = 15 cells; N = 5 mice; p = 4.47×10 −13 versus WT) and KO+AAV-hSyn-EGFP ( : 5.15 ± 1.61%; n = 12 cells; N = 4 mice; p = 0.89 versus KO). Right panel, IPSC traces collected before and during DSI. Data are represented as mean ± SEM. ∗ p < 0.05; ∗∗∗ p < 0.001, one-way ANOVA with Bonferroni’s multiple comparisons test.
Aav Cre, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/105545+serotype+aav9/pmc09599210-42-25-31?v=Addgene+inc
Average 96 stars, based on 1 article reviews
aav cre - by Bioz Stars, 2026-08
96/100 stars
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100843 aav9  (Addgene inc)
96
Addgene inc 100843 aav9
Expression of FABP5 in astrocytes of FABP5 KO mice rescues DSI (A) AAV-mediated expression of FABP5 under the control of the <t>gfaABC1D</t> promoter in FABP5 KO mice. a1-a3, Immunostaining of FABP5, s100β and merge. Scale bar: 50 μm. a4, Co-localization of FABP5 with s100β. a5, Lack of co-localization of FABP5 with NeuN. Scale bars: 10 μm. (B) Expression of FABP5 in astrocytes rescues DSI in FABP5 KO mice. Left panel, averaged magnitude of DSI obtained in WT ( : 42.23 ± 3.44%; n = 12 cells; N = 3 mice), KO ( : 2.65 ± 1.03%; n = 13 cells; N = 3 mice; p = 3.28×10 −13 versus WT), KO+AAV-gfaABC1D-FABP5 ( : 33.37 ± 3.50%; n = 13 cells; N = 4 mice; p = 0.12 versus WT), and KO+AAV-GFAP-EGFP ( : 6.07 ± 1.58%; n = 12 cells; N = 4 mice; p = 1.71×10 − 8 versus KO+AAV-gfaABC1D-FABP5). Right panel, IPSC traces collected before and during DSI from WT ( ), KO ( ), KO+AAV-gfaABC1D-FABP5 ( ), and KO+AAV-GFAP-EGFP ( ). Scale bars: 100 ms, 200 pA. (C) AAV-mediated expression of FABP5 under the neuron-specific hsynapsin1 promoter in FABP5 KO mice. c1-c3, Immunostaining for NeuN, FABP5, and merge. Scale bar: 50 μm. c4, Co-localization of FABP5 with NeuN. c5, Lack of co-localization of FABP5 with s100β. Scale bars: 10 μm. (D) Expression of FABP5 in neurons partially rescues DSI in FABP5 KO mice. Left panel, Summary of DSI magnitude recorded from WT ( : 39.52 ± 1.65%; n = 11 cells; N = 5 mice), KO ( : 1.43 ± 0.66%; n = 11 cells; N = 4 mice; p = 4.67×10 −18 versus WT), KO+AAV-hSyn-FABP5 ( : 13.95 ± 2.13%; n = 15 cells; N = 5 mice; p = 4.47×10 −13 versus WT) and KO+AAV-hSyn-EGFP ( : 5.15 ± 1.61%; n = 12 cells; N = 4 mice; p = 0.89 versus KO). Right panel, IPSC traces collected before and during DSI. Data are represented as mean ± SEM. ∗ p < 0.05; ∗∗∗ p < 0.001, one-way ANOVA with Bonferroni’s multiple comparisons test.
100843 Aav9, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/105545+serotype+aav9/pmc07310222__Suppl_TableS1___S5-5-96-124?v=Addgene+inc
Average 96 stars, based on 1 article reviews
100843 aav9 - by Bioz Stars, 2026-08
96/100 stars
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cre recombinase  (Addgene inc)
96
Addgene inc cre recombinase
Expression of FABP5 in astrocytes of FABP5 KO mice rescues DSI (A) AAV-mediated expression of FABP5 under the control of the <t>gfaABC1D</t> promoter in FABP5 KO mice. a1-a3, Immunostaining of FABP5, s100β and merge. Scale bar: 50 μm. a4, Co-localization of FABP5 with s100β. a5, Lack of co-localization of FABP5 with NeuN. Scale bars: 10 μm. (B) Expression of FABP5 in astrocytes rescues DSI in FABP5 KO mice. Left panel, averaged magnitude of DSI obtained in WT ( : 42.23 ± 3.44%; n = 12 cells; N = 3 mice), KO ( : 2.65 ± 1.03%; n = 13 cells; N = 3 mice; p = 3.28×10 −13 versus WT), KO+AAV-gfaABC1D-FABP5 ( : 33.37 ± 3.50%; n = 13 cells; N = 4 mice; p = 0.12 versus WT), and KO+AAV-GFAP-EGFP ( : 6.07 ± 1.58%; n = 12 cells; N = 4 mice; p = 1.71×10 − 8 versus KO+AAV-gfaABC1D-FABP5). Right panel, IPSC traces collected before and during DSI from WT ( ), KO ( ), KO+AAV-gfaABC1D-FABP5 ( ), and KO+AAV-GFAP-EGFP ( ). Scale bars: 100 ms, 200 pA. (C) AAV-mediated expression of FABP5 under the neuron-specific hsynapsin1 promoter in FABP5 KO mice. c1-c3, Immunostaining for NeuN, FABP5, and merge. Scale bar: 50 μm. c4, Co-localization of FABP5 with NeuN. c5, Lack of co-localization of FABP5 with s100β. Scale bars: 10 μm. (D) Expression of FABP5 in neurons partially rescues DSI in FABP5 KO mice. Left panel, Summary of DSI magnitude recorded from WT ( : 39.52 ± 1.65%; n = 11 cells; N = 5 mice), KO ( : 1.43 ± 0.66%; n = 11 cells; N = 4 mice; p = 4.67×10 −18 versus WT), KO+AAV-hSyn-FABP5 ( : 13.95 ± 2.13%; n = 15 cells; N = 5 mice; p = 4.47×10 −13 versus WT) and KO+AAV-hSyn-EGFP ( : 5.15 ± 1.61%; n = 12 cells; N = 4 mice; p = 0.89 versus KO). Right panel, IPSC traces collected before and during DSI. Data are represented as mean ± SEM. ∗ p < 0.05; ∗∗∗ p < 0.001, one-way ANOVA with Bonferroni’s multiple comparisons test.
Cre Recombinase, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/105545+serotype+aav9/pmc09599210-42-14-21?v=Addgene+inc
Average 96 stars, based on 1 article reviews
cre recombinase - by Bioz Stars, 2026-08
96/100 stars
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51502 aav9  (Addgene inc)
96
Addgene inc 51502 aav9
Expression of FABP5 in astrocytes of FABP5 KO mice rescues DSI (A) AAV-mediated expression of FABP5 under the control of the <t>gfaABC1D</t> promoter in FABP5 KO mice. a1-a3, Immunostaining of FABP5, s100β and merge. Scale bar: 50 μm. a4, Co-localization of FABP5 with s100β. a5, Lack of co-localization of FABP5 with NeuN. Scale bars: 10 μm. (B) Expression of FABP5 in astrocytes rescues DSI in FABP5 KO mice. Left panel, averaged magnitude of DSI obtained in WT ( : 42.23 ± 3.44%; n = 12 cells; N = 3 mice), KO ( : 2.65 ± 1.03%; n = 13 cells; N = 3 mice; p = 3.28×10 −13 versus WT), KO+AAV-gfaABC1D-FABP5 ( : 33.37 ± 3.50%; n = 13 cells; N = 4 mice; p = 0.12 versus WT), and KO+AAV-GFAP-EGFP ( : 6.07 ± 1.58%; n = 12 cells; N = 4 mice; p = 1.71×10 − 8 versus KO+AAV-gfaABC1D-FABP5). Right panel, IPSC traces collected before and during DSI from WT ( ), KO ( ), KO+AAV-gfaABC1D-FABP5 ( ), and KO+AAV-GFAP-EGFP ( ). Scale bars: 100 ms, 200 pA. (C) AAV-mediated expression of FABP5 under the neuron-specific hsynapsin1 promoter in FABP5 KO mice. c1-c3, Immunostaining for NeuN, FABP5, and merge. Scale bar: 50 μm. c4, Co-localization of FABP5 with NeuN. c5, Lack of co-localization of FABP5 with s100β. Scale bars: 10 μm. (D) Expression of FABP5 in neurons partially rescues DSI in FABP5 KO mice. Left panel, Summary of DSI magnitude recorded from WT ( : 39.52 ± 1.65%; n = 11 cells; N = 5 mice), KO ( : 1.43 ± 0.66%; n = 11 cells; N = 4 mice; p = 4.67×10 −18 versus WT), KO+AAV-hSyn-FABP5 ( : 13.95 ± 2.13%; n = 15 cells; N = 5 mice; p = 4.47×10 −13 versus WT) and KO+AAV-hSyn-EGFP ( : 5.15 ± 1.61%; n = 12 cells; N = 4 mice; p = 0.89 versus KO). Right panel, IPSC traces collected before and during DSI. Data are represented as mean ± SEM. ∗ p < 0.05; ∗∗∗ p < 0.001, one-way ANOVA with Bonferroni’s multiple comparisons test.
51502 Aav9, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/105545+serotype+aav9/pmc07310222__Suppl_TableS1___S5-5-51-124?v=Addgene+inc
Average 96 stars, based on 1 article reviews
51502 aav9 - by Bioz Stars, 2026-08
96/100 stars
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108912 aav9  (Addgene inc)
93
Addgene inc 108912 aav9
Expression of FABP5 in astrocytes of FABP5 KO mice rescues DSI (A) AAV-mediated expression of FABP5 under the control of the <t>gfaABC1D</t> promoter in FABP5 KO mice. a1-a3, Immunostaining of FABP5, s100β and merge. Scale bar: 50 μm. a4, Co-localization of FABP5 with s100β. a5, Lack of co-localization of FABP5 with NeuN. Scale bars: 10 μm. (B) Expression of FABP5 in astrocytes rescues DSI in FABP5 KO mice. Left panel, averaged magnitude of DSI obtained in WT ( : 42.23 ± 3.44%; n = 12 cells; N = 3 mice), KO ( : 2.65 ± 1.03%; n = 13 cells; N = 3 mice; p = 3.28×10 −13 versus WT), KO+AAV-gfaABC1D-FABP5 ( : 33.37 ± 3.50%; n = 13 cells; N = 4 mice; p = 0.12 versus WT), and KO+AAV-GFAP-EGFP ( : 6.07 ± 1.58%; n = 12 cells; N = 4 mice; p = 1.71×10 − 8 versus KO+AAV-gfaABC1D-FABP5). Right panel, IPSC traces collected before and during DSI from WT ( ), KO ( ), KO+AAV-gfaABC1D-FABP5 ( ), and KO+AAV-GFAP-EGFP ( ). Scale bars: 100 ms, 200 pA. (C) AAV-mediated expression of FABP5 under the neuron-specific hsynapsin1 promoter in FABP5 KO mice. c1-c3, Immunostaining for NeuN, FABP5, and merge. Scale bar: 50 μm. c4, Co-localization of FABP5 with NeuN. c5, Lack of co-localization of FABP5 with s100β. Scale bars: 10 μm. (D) Expression of FABP5 in neurons partially rescues DSI in FABP5 KO mice. Left panel, Summary of DSI magnitude recorded from WT ( : 39.52 ± 1.65%; n = 11 cells; N = 5 mice), KO ( : 1.43 ± 0.66%; n = 11 cells; N = 4 mice; p = 4.67×10 −18 versus WT), KO+AAV-hSyn-FABP5 ( : 13.95 ± 2.13%; n = 15 cells; N = 5 mice; p = 4.47×10 −13 versus WT) and KO+AAV-hSyn-EGFP ( : 5.15 ± 1.61%; n = 12 cells; N = 4 mice; p = 0.89 versus KO). Right panel, IPSC traces collected before and during DSI. Data are represented as mean ± SEM. ∗ p < 0.05; ∗∗∗ p < 0.001, one-way ANOVA with Bonferroni’s multiple comparisons test.
108912 Aav9, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/105545+serotype+aav9/pmc07310222__Suppl_TableS1___S5-5-57-124?v=Addgene+inc
Average 93 stars, based on 1 article reviews
108912 aav9 - by Bioz Stars, 2026-08
93/100 stars
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107787 aav8  (Addgene inc)
96
Addgene inc 107787 aav8
Expression of FABP5 in astrocytes of FABP5 KO mice rescues DSI (A) AAV-mediated expression of FABP5 under the control of the <t>gfaABC1D</t> promoter in FABP5 KO mice. a1-a3, Immunostaining of FABP5, s100β and merge. Scale bar: 50 μm. a4, Co-localization of FABP5 with s100β. a5, Lack of co-localization of FABP5 with NeuN. Scale bars: 10 μm. (B) Expression of FABP5 in astrocytes rescues DSI in FABP5 KO mice. Left panel, averaged magnitude of DSI obtained in WT ( : 42.23 ± 3.44%; n = 12 cells; N = 3 mice), KO ( : 2.65 ± 1.03%; n = 13 cells; N = 3 mice; p = 3.28×10 −13 versus WT), KO+AAV-gfaABC1D-FABP5 ( : 33.37 ± 3.50%; n = 13 cells; N = 4 mice; p = 0.12 versus WT), and KO+AAV-GFAP-EGFP ( : 6.07 ± 1.58%; n = 12 cells; N = 4 mice; p = 1.71×10 − 8 versus KO+AAV-gfaABC1D-FABP5). Right panel, IPSC traces collected before and during DSI from WT ( ), KO ( ), KO+AAV-gfaABC1D-FABP5 ( ), and KO+AAV-GFAP-EGFP ( ). Scale bars: 100 ms, 200 pA. (C) AAV-mediated expression of FABP5 under the neuron-specific hsynapsin1 promoter in FABP5 KO mice. c1-c3, Immunostaining for NeuN, FABP5, and merge. Scale bar: 50 μm. c4, Co-localization of FABP5 with NeuN. c5, Lack of co-localization of FABP5 with s100β. Scale bars: 10 μm. (D) Expression of FABP5 in neurons partially rescues DSI in FABP5 KO mice. Left panel, Summary of DSI magnitude recorded from WT ( : 39.52 ± 1.65%; n = 11 cells; N = 5 mice), KO ( : 1.43 ± 0.66%; n = 11 cells; N = 4 mice; p = 4.67×10 −18 versus WT), KO+AAV-hSyn-FABP5 ( : 13.95 ± 2.13%; n = 15 cells; N = 5 mice; p = 4.47×10 −13 versus WT) and KO+AAV-hSyn-EGFP ( : 5.15 ± 1.61%; n = 12 cells; N = 4 mice; p = 0.89 versus KO). Right panel, IPSC traces collected before and during DSI. Data are represented as mean ± SEM. ∗ p < 0.05; ∗∗∗ p < 0.001, one-way ANOVA with Bonferroni’s multiple comparisons test.
107787 Aav8, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/105545+serotype+aav9/pmc07310222__Suppl_TableS1___S5-5-93-124?v=Addgene+inc
Average 96 stars, based on 1 article reviews
107787 aav8 - by Bioz Stars, 2026-08
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99130 aav1  (Addgene inc)
95
Addgene inc 99130 aav1
Expression of FABP5 in astrocytes of FABP5 KO mice rescues DSI (A) AAV-mediated expression of FABP5 under the control of the <t>gfaABC1D</t> promoter in FABP5 KO mice. a1-a3, Immunostaining of FABP5, s100β and merge. Scale bar: 50 μm. a4, Co-localization of FABP5 with s100β. a5, Lack of co-localization of FABP5 with NeuN. Scale bars: 10 μm. (B) Expression of FABP5 in astrocytes rescues DSI in FABP5 KO mice. Left panel, averaged magnitude of DSI obtained in WT ( : 42.23 ± 3.44%; n = 12 cells; N = 3 mice), KO ( : 2.65 ± 1.03%; n = 13 cells; N = 3 mice; p = 3.28×10 −13 versus WT), KO+AAV-gfaABC1D-FABP5 ( : 33.37 ± 3.50%; n = 13 cells; N = 4 mice; p = 0.12 versus WT), and KO+AAV-GFAP-EGFP ( : 6.07 ± 1.58%; n = 12 cells; N = 4 mice; p = 1.71×10 − 8 versus KO+AAV-gfaABC1D-FABP5). Right panel, IPSC traces collected before and during DSI from WT ( ), KO ( ), KO+AAV-gfaABC1D-FABP5 ( ), and KO+AAV-GFAP-EGFP ( ). Scale bars: 100 ms, 200 pA. (C) AAV-mediated expression of FABP5 under the neuron-specific hsynapsin1 promoter in FABP5 KO mice. c1-c3, Immunostaining for NeuN, FABP5, and merge. Scale bar: 50 μm. c4, Co-localization of FABP5 with NeuN. c5, Lack of co-localization of FABP5 with s100β. Scale bars: 10 μm. (D) Expression of FABP5 in neurons partially rescues DSI in FABP5 KO mice. Left panel, Summary of DSI magnitude recorded from WT ( : 39.52 ± 1.65%; n = 11 cells; N = 5 mice), KO ( : 1.43 ± 0.66%; n = 11 cells; N = 4 mice; p = 4.67×10 −18 versus WT), KO+AAV-hSyn-FABP5 ( : 13.95 ± 2.13%; n = 15 cells; N = 5 mice; p = 4.47×10 −13 versus WT) and KO+AAV-hSyn-EGFP ( : 5.15 ± 1.61%; n = 12 cells; N = 4 mice; p = 0.89 versus KO). Right panel, IPSC traces collected before and during DSI. Data are represented as mean ± SEM. ∗ p < 0.05; ∗∗∗ p < 0.001, one-way ANOVA with Bonferroni’s multiple comparisons test.
99130 Aav1, supplied by Addgene inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/105545+serotype+aav9/pmc07310222__Suppl_TableS1___S5-5-102-124?v=Addgene+inc
Average 95 stars, based on 1 article reviews
99130 aav1 - by Bioz Stars, 2026-08
95/100 stars
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114472 aav2  (Addgene inc)
97
Addgene inc 114472 aav2
Expression of FABP5 in astrocytes of FABP5 KO mice rescues DSI (A) AAV-mediated expression of FABP5 under the control of the <t>gfaABC1D</t> promoter in FABP5 KO mice. a1-a3, Immunostaining of FABP5, s100β and merge. Scale bar: 50 μm. a4, Co-localization of FABP5 with s100β. a5, Lack of co-localization of FABP5 with NeuN. Scale bars: 10 μm. (B) Expression of FABP5 in astrocytes rescues DSI in FABP5 KO mice. Left panel, averaged magnitude of DSI obtained in WT ( : 42.23 ± 3.44%; n = 12 cells; N = 3 mice), KO ( : 2.65 ± 1.03%; n = 13 cells; N = 3 mice; p = 3.28×10 −13 versus WT), KO+AAV-gfaABC1D-FABP5 ( : 33.37 ± 3.50%; n = 13 cells; N = 4 mice; p = 0.12 versus WT), and KO+AAV-GFAP-EGFP ( : 6.07 ± 1.58%; n = 12 cells; N = 4 mice; p = 1.71×10 − 8 versus KO+AAV-gfaABC1D-FABP5). Right panel, IPSC traces collected before and during DSI from WT ( ), KO ( ), KO+AAV-gfaABC1D-FABP5 ( ), and KO+AAV-GFAP-EGFP ( ). Scale bars: 100 ms, 200 pA. (C) AAV-mediated expression of FABP5 under the neuron-specific hsynapsin1 promoter in FABP5 KO mice. c1-c3, Immunostaining for NeuN, FABP5, and merge. Scale bar: 50 μm. c4, Co-localization of FABP5 with NeuN. c5, Lack of co-localization of FABP5 with s100β. Scale bars: 10 μm. (D) Expression of FABP5 in neurons partially rescues DSI in FABP5 KO mice. Left panel, Summary of DSI magnitude recorded from WT ( : 39.52 ± 1.65%; n = 11 cells; N = 5 mice), KO ( : 1.43 ± 0.66%; n = 11 cells; N = 4 mice; p = 4.67×10 −18 versus WT), KO+AAV-hSyn-FABP5 ( : 13.95 ± 2.13%; n = 15 cells; N = 5 mice; p = 4.47×10 −13 versus WT) and KO+AAV-hSyn-EGFP ( : 5.15 ± 1.61%; n = 12 cells; N = 4 mice; p = 0.89 versus KO). Right panel, IPSC traces collected before and during DSI. Data are represented as mean ± SEM. ∗ p < 0.05; ∗∗∗ p < 0.001, one-way ANOVA with Bonferroni’s multiple comparisons test.
114472 Aav2, supplied by Addgene inc, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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112164 aav1  (Addgene inc)
93
Addgene inc 112164 aav1
Expression of FABP5 in astrocytes of FABP5 KO mice rescues DSI (A) AAV-mediated expression of FABP5 under the control of the <t>gfaABC1D</t> promoter in FABP5 KO mice. a1-a3, Immunostaining of FABP5, s100β and merge. Scale bar: 50 μm. a4, Co-localization of FABP5 with s100β. a5, Lack of co-localization of FABP5 with NeuN. Scale bars: 10 μm. (B) Expression of FABP5 in astrocytes rescues DSI in FABP5 KO mice. Left panel, averaged magnitude of DSI obtained in WT ( : 42.23 ± 3.44%; n = 12 cells; N = 3 mice), KO ( : 2.65 ± 1.03%; n = 13 cells; N = 3 mice; p = 3.28×10 −13 versus WT), KO+AAV-gfaABC1D-FABP5 ( : 33.37 ± 3.50%; n = 13 cells; N = 4 mice; p = 0.12 versus WT), and KO+AAV-GFAP-EGFP ( : 6.07 ± 1.58%; n = 12 cells; N = 4 mice; p = 1.71×10 − 8 versus KO+AAV-gfaABC1D-FABP5). Right panel, IPSC traces collected before and during DSI from WT ( ), KO ( ), KO+AAV-gfaABC1D-FABP5 ( ), and KO+AAV-GFAP-EGFP ( ). Scale bars: 100 ms, 200 pA. (C) AAV-mediated expression of FABP5 under the neuron-specific hsynapsin1 promoter in FABP5 KO mice. c1-c3, Immunostaining for NeuN, FABP5, and merge. Scale bar: 50 μm. c4, Co-localization of FABP5 with NeuN. c5, Lack of co-localization of FABP5 with s100β. Scale bars: 10 μm. (D) Expression of FABP5 in neurons partially rescues DSI in FABP5 KO mice. Left panel, Summary of DSI magnitude recorded from WT ( : 39.52 ± 1.65%; n = 11 cells; N = 5 mice), KO ( : 1.43 ± 0.66%; n = 11 cells; N = 4 mice; p = 4.67×10 −18 versus WT), KO+AAV-hSyn-FABP5 ( : 13.95 ± 2.13%; n = 15 cells; N = 5 mice; p = 4.47×10 −13 versus WT) and KO+AAV-hSyn-EGFP ( : 5.15 ± 1.61%; n = 12 cells; N = 4 mice; p = 0.89 versus KO). Right panel, IPSC traces collected before and during DSI. Data are represented as mean ± SEM. ∗ p < 0.05; ∗∗∗ p < 0.001, one-way ANOVA with Bonferroni’s multiple comparisons test.
112164 Aav1, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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105539 aav1  (Addgene inc)
94
Addgene inc 105539 aav1
Expression of FABP5 in astrocytes of FABP5 KO mice rescues DSI (A) AAV-mediated expression of FABP5 under the control of the <t>gfaABC1D</t> promoter in FABP5 KO mice. a1-a3, Immunostaining of FABP5, s100β and merge. Scale bar: 50 μm. a4, Co-localization of FABP5 with s100β. a5, Lack of co-localization of FABP5 with NeuN. Scale bars: 10 μm. (B) Expression of FABP5 in astrocytes rescues DSI in FABP5 KO mice. Left panel, averaged magnitude of DSI obtained in WT ( : 42.23 ± 3.44%; n = 12 cells; N = 3 mice), KO ( : 2.65 ± 1.03%; n = 13 cells; N = 3 mice; p = 3.28×10 −13 versus WT), KO+AAV-gfaABC1D-FABP5 ( : 33.37 ± 3.50%; n = 13 cells; N = 4 mice; p = 0.12 versus WT), and KO+AAV-GFAP-EGFP ( : 6.07 ± 1.58%; n = 12 cells; N = 4 mice; p = 1.71×10 − 8 versus KO+AAV-gfaABC1D-FABP5). Right panel, IPSC traces collected before and during DSI from WT ( ), KO ( ), KO+AAV-gfaABC1D-FABP5 ( ), and KO+AAV-GFAP-EGFP ( ). Scale bars: 100 ms, 200 pA. (C) AAV-mediated expression of FABP5 under the neuron-specific hsynapsin1 promoter in FABP5 KO mice. c1-c3, Immunostaining for NeuN, FABP5, and merge. Scale bar: 50 μm. c4, Co-localization of FABP5 with NeuN. c5, Lack of co-localization of FABP5 with s100β. Scale bars: 10 μm. (D) Expression of FABP5 in neurons partially rescues DSI in FABP5 KO mice. Left panel, Summary of DSI magnitude recorded from WT ( : 39.52 ± 1.65%; n = 11 cells; N = 5 mice), KO ( : 1.43 ± 0.66%; n = 11 cells; N = 4 mice; p = 4.67×10 −18 versus WT), KO+AAV-hSyn-FABP5 ( : 13.95 ± 2.13%; n = 15 cells; N = 5 mice; p = 4.47×10 −13 versus WT) and KO+AAV-hSyn-EGFP ( : 5.15 ± 1.61%; n = 12 cells; N = 4 mice; p = 0.89 versus KO). Right panel, IPSC traces collected before and during DSI. Data are represented as mean ± SEM. ∗ p < 0.05; ∗∗∗ p < 0.001, one-way ANOVA with Bonferroni’s multiple comparisons test.
105539 Aav1, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


The effect of genotype on the pain sensitivity determined by tail-flick test ( A , B ). The influence of genotype ( A ) and the influence of AAV-Cre injection ( B ) on thermal pain sensitivity. Data are the mean ± SEM. n = 5–14 ( A ), n = 8 ( B ). +/+, wild type; +/flox, floxed hetero; flox/flox, floxed homo. The effect of knockdown of RTP4 in PVN on the development of antinociceptive tolerance to morphine ( C ). The daily changes in antinociceptive effect of morphine during repeated administration. Data are the mean ± SEM. n = 7 (AAV-eGFP) (control), n = 10 (AAV-Cre), **** p < 0.0001 vs. Day1 (AAV-eGFP); # p < 0.05, #### p < 0.0001 vs. Day1 (AAV-Cre), Dunnett’s multiple comparison test; † p < 0.05, †† p < 0.01 vs. AAV-eGFP (control), repeated measures ANOVA and the post-hoc Tukey’s multiple comparisons test. The effect of AAV-Cre on RTP4 expression in PVN area (D and E). Representative images of the immunohistochemical signal for RTP4 and the AAV-eGFP signal of the virus infected in the PVN area. Arrows indicate the eGFP-positive cell. The mean intensity of the RTP4 signal in eGFP-positive cells were measured by Image J software, as described in the Methods section (E). The mean signal intensity of RTP4 in the RTP4/eGFP double-positive cells were measured from a single cryostat brain section, including the PVN region from 7 (AAV-eGFP) and 6 (AAV-Cre) mice. Ten to 40 eGFP-positive cells were measured from each section. Data are the mean ± SEM. n = 7 (AAV-eGFP) (control), n = 6 (AAV-Cre), * p < 0.05 unpaired t -test.

Journal: Biomolecules

Article Title: Receptor Transporter Protein 4 (RTP4) in the Hypothalamus Is Involved in the Development of Antinociceptive Tolerance to Morphine

doi: 10.3390/biom12101471

Figure Lengend Snippet: The effect of genotype on the pain sensitivity determined by tail-flick test ( A , B ). The influence of genotype ( A ) and the influence of AAV-Cre injection ( B ) on thermal pain sensitivity. Data are the mean ± SEM. n = 5–14 ( A ), n = 8 ( B ). +/+, wild type; +/flox, floxed hetero; flox/flox, floxed homo. The effect of knockdown of RTP4 in PVN on the development of antinociceptive tolerance to morphine ( C ). The daily changes in antinociceptive effect of morphine during repeated administration. Data are the mean ± SEM. n = 7 (AAV-eGFP) (control), n = 10 (AAV-Cre), **** p < 0.0001 vs. Day1 (AAV-eGFP); # p < 0.05, #### p < 0.0001 vs. Day1 (AAV-Cre), Dunnett’s multiple comparison test; † p < 0.05, †† p < 0.01 vs. AAV-eGFP (control), repeated measures ANOVA and the post-hoc Tukey’s multiple comparisons test. The effect of AAV-Cre on RTP4 expression in PVN area (D and E). Representative images of the immunohistochemical signal for RTP4 and the AAV-eGFP signal of the virus infected in the PVN area. Arrows indicate the eGFP-positive cell. The mean intensity of the RTP4 signal in eGFP-positive cells were measured by Image J software, as described in the Methods section (E). The mean signal intensity of RTP4 in the RTP4/eGFP double-positive cells were measured from a single cryostat brain section, including the PVN region from 7 (AAV-eGFP) and 6 (AAV-Cre) mice. Ten to 40 eGFP-positive cells were measured from each section. Data are the mean ± SEM. n = 7 (AAV-eGFP) (control), n = 6 (AAV-Cre), * p < 0.05 unpaired t -test.

Article Snippet: To ablate the Rtp4 gene in a PVN-specific manner, an AAV vector expressing CMV-driven Cre recombinase, fused to eGFP (Serotype: AAV9: Addgene.org/105545, Watertown, MA, USA) (AAV-Cre) or eGFP alone (Serotype: AAV9: Addgene.org/105530) (AAV-eGFP), as a control, was bilaterally injected into the PVN, as described below.

Techniques: Tail Flick Test, Injection, Knockdown, Control, Comparison, Expressing, Immunohistochemical staining, Virus, Infection, Software

Expression of FABP5 in astrocytes of FABP5 KO mice rescues DSI (A) AAV-mediated expression of FABP5 under the control of the gfaABC1D promoter in FABP5 KO mice. a1-a3, Immunostaining of FABP5, s100β and merge. Scale bar: 50 μm. a4, Co-localization of FABP5 with s100β. a5, Lack of co-localization of FABP5 with NeuN. Scale bars: 10 μm. (B) Expression of FABP5 in astrocytes rescues DSI in FABP5 KO mice. Left panel, averaged magnitude of DSI obtained in WT ( : 42.23 ± 3.44%; n = 12 cells; N = 3 mice), KO ( : 2.65 ± 1.03%; n = 13 cells; N = 3 mice; p = 3.28×10 −13 versus WT), KO+AAV-gfaABC1D-FABP5 ( : 33.37 ± 3.50%; n = 13 cells; N = 4 mice; p = 0.12 versus WT), and KO+AAV-GFAP-EGFP ( : 6.07 ± 1.58%; n = 12 cells; N = 4 mice; p = 1.71×10 − 8 versus KO+AAV-gfaABC1D-FABP5). Right panel, IPSC traces collected before and during DSI from WT ( ), KO ( ), KO+AAV-gfaABC1D-FABP5 ( ), and KO+AAV-GFAP-EGFP ( ). Scale bars: 100 ms, 200 pA. (C) AAV-mediated expression of FABP5 under the neuron-specific hsynapsin1 promoter in FABP5 KO mice. c1-c3, Immunostaining for NeuN, FABP5, and merge. Scale bar: 50 μm. c4, Co-localization of FABP5 with NeuN. c5, Lack of co-localization of FABP5 with s100β. Scale bars: 10 μm. (D) Expression of FABP5 in neurons partially rescues DSI in FABP5 KO mice. Left panel, Summary of DSI magnitude recorded from WT ( : 39.52 ± 1.65%; n = 11 cells; N = 5 mice), KO ( : 1.43 ± 0.66%; n = 11 cells; N = 4 mice; p = 4.67×10 −18 versus WT), KO+AAV-hSyn-FABP5 ( : 13.95 ± 2.13%; n = 15 cells; N = 5 mice; p = 4.47×10 −13 versus WT) and KO+AAV-hSyn-EGFP ( : 5.15 ± 1.61%; n = 12 cells; N = 4 mice; p = 0.89 versus KO). Right panel, IPSC traces collected before and during DSI. Data are represented as mean ± SEM. ∗ p < 0.05; ∗∗∗ p < 0.001, one-way ANOVA with Bonferroni’s multiple comparisons test.

Journal: iScience

Article Title: Astrocytic FABP5 mediates retrograde endocannabinoid transport at central synapses

doi: 10.1016/j.isci.2025.112342

Figure Lengend Snippet: Expression of FABP5 in astrocytes of FABP5 KO mice rescues DSI (A) AAV-mediated expression of FABP5 under the control of the gfaABC1D promoter in FABP5 KO mice. a1-a3, Immunostaining of FABP5, s100β and merge. Scale bar: 50 μm. a4, Co-localization of FABP5 with s100β. a5, Lack of co-localization of FABP5 with NeuN. Scale bars: 10 μm. (B) Expression of FABP5 in astrocytes rescues DSI in FABP5 KO mice. Left panel, averaged magnitude of DSI obtained in WT ( : 42.23 ± 3.44%; n = 12 cells; N = 3 mice), KO ( : 2.65 ± 1.03%; n = 13 cells; N = 3 mice; p = 3.28×10 −13 versus WT), KO+AAV-gfaABC1D-FABP5 ( : 33.37 ± 3.50%; n = 13 cells; N = 4 mice; p = 0.12 versus WT), and KO+AAV-GFAP-EGFP ( : 6.07 ± 1.58%; n = 12 cells; N = 4 mice; p = 1.71×10 − 8 versus KO+AAV-gfaABC1D-FABP5). Right panel, IPSC traces collected before and during DSI from WT ( ), KO ( ), KO+AAV-gfaABC1D-FABP5 ( ), and KO+AAV-GFAP-EGFP ( ). Scale bars: 100 ms, 200 pA. (C) AAV-mediated expression of FABP5 under the neuron-specific hsynapsin1 promoter in FABP5 KO mice. c1-c3, Immunostaining for NeuN, FABP5, and merge. Scale bar: 50 μm. c4, Co-localization of FABP5 with NeuN. c5, Lack of co-localization of FABP5 with s100β. Scale bars: 10 μm. (D) Expression of FABP5 in neurons partially rescues DSI in FABP5 KO mice. Left panel, Summary of DSI magnitude recorded from WT ( : 39.52 ± 1.65%; n = 11 cells; N = 5 mice), KO ( : 1.43 ± 0.66%; n = 11 cells; N = 4 mice; p = 4.67×10 −18 versus WT), KO+AAV-hSyn-FABP5 ( : 13.95 ± 2.13%; n = 15 cells; N = 5 mice; p = 4.47×10 −13 versus WT) and KO+AAV-hSyn-EGFP ( : 5.15 ± 1.61%; n = 12 cells; N = 4 mice; p = 0.89 versus KO). Right panel, IPSC traces collected before and during DSI. Data are represented as mean ± SEM. ∗ p < 0.05; ∗∗∗ p < 0.001, one-way ANOVA with Bonferroni’s multiple comparisons test.

Article Snippet: The following AAVs were used: AAV-CAG-FABP5 expressing mouse FABP5 under the CAG promoter (Duke VVC #pBK366, serotype AAV9), AAV-CAG-FABP5 MUT expressing FABP5 MUT (Duke VVC #1937, serotype AAV9), AAV-CAG-FABP5 SEC expressing FABP5 SEC (Duke VVC #pBK383, serotype AAV9), AAV-CAG-FABP7 expressing mouse FABP7 (Duke VVC #pBK1433, serotype AAV9), AAV-CMV-Cre (Addgene, #105545, serotype AAV9), AAV-CMV-GFP (Addgene, #105530, serotype AAV9), AAV-hSyn-Cre (Addgene, #105540, serotype AAV5), pAAV-hSyn-EGFP (Addgene, #50465, serotype AAV5), AAV-gfaABC1D-FABP5 expressing FABP5 under the astrocyte-specific gfaABC1D promoter (Duke VVC #pBK1762, serotype AAV8), pAAV.GFAP.eGFP.WPRE.hGH (Addgene, #105549, serotype AAV5), and AAV-hSyn-FABP5 (Duke VVC #pBK1761, serotype AAV5).

Techniques: Expressing, Control, Immunostaining

Journal: iScience

Article Title: Astrocytic FABP5 mediates retrograde endocannabinoid transport at central synapses

doi: 10.1016/j.isci.2025.112342

Figure Lengend Snippet:

Article Snippet: The following AAVs were used: AAV-CAG-FABP5 expressing mouse FABP5 under the CAG promoter (Duke VVC #pBK366, serotype AAV9), AAV-CAG-FABP5 MUT expressing FABP5 MUT (Duke VVC #1937, serotype AAV9), AAV-CAG-FABP5 SEC expressing FABP5 SEC (Duke VVC #pBK383, serotype AAV9), AAV-CAG-FABP7 expressing mouse FABP7 (Duke VVC #pBK1433, serotype AAV9), AAV-CMV-Cre (Addgene, #105545, serotype AAV9), AAV-CMV-GFP (Addgene, #105530, serotype AAV9), AAV-hSyn-Cre (Addgene, #105540, serotype AAV5), pAAV-hSyn-EGFP (Addgene, #50465, serotype AAV5), AAV-gfaABC1D-FABP5 expressing FABP5 under the astrocyte-specific gfaABC1D promoter (Duke VVC #pBK1762, serotype AAV8), pAAV.GFAP.eGFP.WPRE.hGH (Addgene, #105549, serotype AAV5), and AAV-hSyn-FABP5 (Duke VVC #pBK1761, serotype AAV5).

Techniques: Virus, Recombinant, Software, Imaging, Transfection, Real-time Polymerase Chain Reaction, SYBR Green Assay, Mass Spectrometry, Control, Microscopy